Recombinant INSM1 antibody (AA 81-125)
Quick Overview for Recombinant INSM1 antibody (AA 81-125) (ABIN7477503)
Target
See all INSM1 AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 81-125
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Immunogen
- Recombinant fragment (around aa81-125) corresponding to the N-terminus of human INSM1
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Isotype
- IgG
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Application Notes
- IHC 1:75-1:150
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Comment
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Positive Control: Pancreas: Islet cells should show a moderate to strong INSM1 immunostaining
Negative Control: Pancreas: All acinar cells should be negative
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- INSM1 (Insulinoma-Associated 1 (INSM1))
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Alternative Name
- INSM1
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Background
- A1, Insulinoma associated protein 1 (IA1), Zinc finger protein IA1,INSM1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- Q01101
Target
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