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Myeloperoxidase antibody

The Mouse Monoclonal anti-Myeloperoxidase antibody has been validated for IHC. It is suitable to detect Myeloperoxidase in samples from Human.
Catalog No. ABIN7477523

Quick Overview for Myeloperoxidase antibody (ABIN7477523)

Target

See all Myeloperoxidase (MPO) Antibodies
Myeloperoxidase (MPO)

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Myeloperoxidase antibody is un-conjugated

Application

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Immunohistochemistry (IHC)

Clone

MSVA-692M
  • Immunogen

    Recombinant fragment of human MPO protein (exact sequence is proprietary)

    Isotype

    IgG
  • Application Notes

    IHC 1:100-1:200

    Comment

    Positive Control: Spleen: Numerous granulocytes with strong MPO staining should be seen within the red pulp of the spleen.

    Negative Control: Colon: MPO immunostaining should be absent in all epithelial cells of the colon while MPO positive granulocytes may be seen within capillaries or in the stroma of the lamina propria.

    Protocol

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target

    Myeloperoxidase (MPO)

    Alternative Name

    Myeloperoxidase / MPO

    Background

    4 kDa / 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain,Myeloperoxidase / MPO antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P05164

    Pathways

    Chromatin Binding
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