Myeloperoxidase antibody
Quick Overview for Myeloperoxidase antibody (ABIN7477523)
Target
See all Myeloperoxidase (MPO) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Immunogen
 - Recombinant fragment of human MPO protein (exact sequence is proprietary)
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Isotype
 - IgG
 
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Application Notes
 - IHC 1:100-1:200
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Comment
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Positive Control: Spleen: Numerous granulocytes with strong MPO staining should be seen within the red pulp of the spleen.
Negative Control: Colon: MPO immunostaining should be absent in all epithelial cells of the colon while MPO positive granulocytes may be seen within capillaries or in the stroma of the lamina propria.
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Protocol
 - Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
 - For Research Use only
 
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Format
 - Liquid
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Storage
 - 4 °C,-20 °C,-80 °C
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Storage Comment
 - Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
 
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- Myeloperoxidase (MPO)
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Alternative Name
 - Myeloperoxidase / MPO
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Background
 - 4 kDa / 89 kDa myeloperoxidase, EC 1.11.1.7, EC1.11.2.2, fj80f04, MPO, mpx, myeloid-specific peroxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain,Myeloperoxidase / MPO antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
 - P05164
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Pathways
 - Chromatin Binding
 
Target
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