Recombinant MUC5AC antibody
Quick Overview for Recombinant MUC5AC antibody (ABIN7477527)
Target
See all MUC5AC AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Immunogen
- M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
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Isotype
- IgG
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Gastric mucosa: A strong cytoplasmic MUC5AC staining should be seen in the surface epithelium.
Negative Control: Kidney: MUC5AC staining should be absent.
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Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))
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Alternative Name
- MUC5AC
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Background
- Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A and C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin,MUC5AC antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P98088
Target
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