Recombinant Nucleolin antibody

Catalog No. ABIN7477541

The Rabbit Monoclonal anti-Nucleolin antibody has been validated for IHC. It is suitable to detect Nucleolin in samples from Human.

Quick Overview for Recombinant Nucleolin antibody (ABIN7477541)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-623R

Product Details anti-Nucleolin Antibody

Immunogen: Recombinant full-length human NCL protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.

Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization