Recombinant TCP1 alpha/CCTA antibody (AA 600-680)

Catalog No. ABIN7477549

This anti-TCP1 alpha/CCTA antibody is a Rabbit Monoclonal antibody detecting TCP1 alpha/CCTA in IHC. Suitable for Human.

Quick Overview for Recombinant TCP1 alpha/CCTA antibody (AA 600-680) (ABIN7477549)

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TCP1 alpha/CCTA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-063R

Product Details anti-TCP1 alpha/CCTA Antibody

Binding Specificity: AA 600-680

Immunogen: Recombinant fragment (around aa600-680) of human TP63 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: Virtually all squamous epithelial cells must show a moderate to strong, nuclear staining, while few scattered lymphocytes and endothelial cells must show a at least a weak staining.

Negative Control: Tonsil: The vast majority of lymphocytes should be p63 negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for TCP1 alpha/CCTA

Target: TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))

Alternative Name: p63

Background: Amplified in squamous cell carcinoma (AIS), Chronic ulcerative stomatitis protein (CUSP), EEC3, Keratinocyte transcription factor KET, LMS, NBP, p40, P51/P63, p53 like transcription factor, p53-related protein p63, RHS, SHFM4, TAp63alpha, TP53CP, TP53L, TP63, TP73, TP73L, Transformation-related protein 63, Trp53rp1, Trp6,3, Tumor protein 63, Tumor protein p53-like, tumor protein p73-like,p63 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q9H3D4