PAPPA antibody (AA 351-523)

Catalog No. ABIN7477551

This Mouse Monoclonal antibody specifically detects PAPPA in IHC. It exhibits reactivity toward Human.

Quick Overview for PAPPA antibody (AA 351-523) (ABIN7477551)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-780M

Product Details anti-PAPPA Antibody

Binding Specificity: AA 351-523

Immunogen: Recombinant fragment (within aa 351-523) of human PAPP-A protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Placenta: Trophoplast and chorion cells should show a strong cytoplasmic papp-A staining.

Negative Control: Colon: Papp-A immunostaining should be completely absent in both stromal end epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPP-A

Background: ASBABP2, Aspecific BCL2 ARE binding protein 2, Differentially placenta 1 expressed protein, DIPLA1, IGF-dependent, IGFBP4ase, Insulin-like growth factor-dependent IGF-binding protein 4 protease (IGFBP-4 protease), PAPA, PAPP A, PAPPA1, Pappalysin-1, Pregnancy Associated Plasma Protein A (PAPP-A),PAPP-A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q13219