Recombinant PAX8 antibody

Catalog No. ABIN7477555

This anti-PAX8 antibody is a Rabbit Monoclonal antibody detecting PAX8 in IHC. Suitable for Human.

Quick Overview for Recombinant PAX8 antibody (ABIN7477555)

Target: PAX8 (Paired Box 8 (PAX8))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-708R

Product Details anti-PAX8 Antibody

Immunogen: Recombinant fragment of human PAX8 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Kidney: At least weak to moderate, distinct nuclear staining should be seen in the majority of epithelial cells of the proximal and distal renal tubules, collection ducts, and the parietal epithelial cells of the Bowman's capsule.

Negative Control: Kidney: At least weak to moderate, distinct nuclear staining should be seen in the majority of epithelial cells of the proximal and distal renal tubules, collection ducts, and the parietal epithelial cells of the Bowman's capsule.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8

Background: Paired box gene 8, Paired domain gene 8, PAX8, paired box homeotic gene 8,PAX8 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation