Recombinant PD-L1 antibody

Catalog No. ABIN7477557

This Rabbit Monoclonal antibody specifically detects PD-L1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant PD-L1 antibody (ABIN7477557)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-711R

Product Details anti-PD-L1 Antibody

Immunogen: KLH-conjugated linear peptide corresponding to human PD-L1

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining.

Negative Control: Appendix (negative staining of all epithelial cells)

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1,PD-L1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints