Periostin antibody (AA 193-326)
Quick Overview for Periostin antibody (AA 193-326) (ABIN7470660)
Target
See all Periostin (POSTN) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 193-326
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Purpose
- Periostin antibody (649M)
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Immunogen
- Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)
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Isotype
- IgG2b, kappa
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Application Notes
- IHC 1:100-1:200
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Comment
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Positive Control: Skin: A strong periostin immunostaining should be seen in the skin, forming a subepithelial band of variable thickness.
Product Description: Periostin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Skin: Periostin immunostaining should be lacking in epithelial cells.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Expiry Date
- 24 months
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- Periostin (POSTN)
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Alternative Name
- Periostin
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Background
- Gene Alias: Fasciclin-I like, Osteoblast specific factor 2 (OSF2), PDLPOSTN, Periodontal ligament specific periostin, Periostin isoform thy2 / th4 / thy6 / thy8, Periostin osteoblast specific factor, PN, POSTN
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UniProt
- Q15063
Target
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