Periostin antibody (AA 193-326)

Catalog No. ABIN7477559

The Mouse Monoclonal anti-Periostin antibody has been validated for IHC. It is suitable to detect Periostin in samples from Human.

Quick Overview for Periostin antibody (AA 193-326) (ABIN7477559)

Target: Periostin (POSTN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Periostin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-649M

Product Details anti-Periostin Antibody

Binding Specificity: AA 193-326

Immunogen: Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Skin: A strong periostin immunostaining should be seen in the skin, forming a subepithelial band of variable thickness.

Negative Control: Skin: Periostin immunostaining should be lacking in epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Periostin

Target: Periostin (POSTN)

Alternative Name: Periostin

Background: Fasciclin-I like, Osteoblast specific factor 2 (OSF2), PDLPOSTN, Periodontal ligament specific periostin, Periostin isoform thy2 / th4 / thy6 / thy8, Periostin osteoblast specific factor, PN, POSTN,Periostin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q15063