Recombinant SATB2 antibody (AA 200-300)

Catalog No. ABIN7477575

This Rabbit Monoclonal antibody specifically detects SATB2 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant SATB2 antibody (AA 200-300) (ABIN7477575)

Target: SATB2 (SATB Homeobox 2 (SATB2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SATB2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-702R

Product Details anti-SATB2 Antibody

Binding Specificity: AA 200-300

Immunogen: Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong nuclear staining should be seen in virtually all columnar epithelial cells.

Negative Control: Colon: SATB2 staining should be absent in stromal and smooth muscle cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for SATB2

Target: SATB2 (SATB Homeobox 2 (SATB2))

Alternative Name: SATB2

Background: DNA-binding protein SATB2, GLSS, SATB homeobox 2, Special AT-rich sequence-binding protein 2,SATB2 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q9UPW6