Recombinant S100P antibody (C-Term)

Catalog No. ABIN7477579

This anti-S100P antibody is a Rabbit Monoclonal antibody detecting S100P in IHC. Suitable for Human.

Quick Overview for Recombinant S100P antibody (C-Term) (ABIN7477579)

Target: S100P (S100 Calcium Binding Protein P (S100P))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This S100P antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-480R

Product Details anti-S100P Antibody

Binding Specificity: C-Term

Immunogen: Recombinant fragment corresponding to C-terminal of human S100P protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Urinary bladder: A strong S100P immunostaining should be seen in the urothelium but not in the stroma or muscular cells.

Negative Control: Kidney: S100P immunostaining should be completely absent.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for S100P

Target: S100P (S100 Calcium Binding Protein P (S100P))

Alternative Name: S100P

Background: Migration inducing gene 9 (MIG9), S100E, S100 calcium binding protein P,S100P antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P25815

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins