Recombinant S100A1 antibody

Catalog No. ABIN7477581

This Rabbit Monoclonal antibody specifically detects S100A1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant S100A1 antibody (ABIN7477581)

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This S100A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-490R

Product Details anti-S100A1 Antibody

Immunogen: Recombinant full-length human S100B protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Appendix: Schwann cells of peripheral nerves and adipocytes should show a strong, predominantly cytoplasmic S100B staining.

Negative Control: Appendix: Smooth muscle and epithelial cells must not show any S100B staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100

Background: NEF, Protein S100-B, S-100 protein beta chain, S100 calcium binding protein beta (neural), S100 calcium-binding protein B, S100 protein beta chain, S100B, S100beta,S100 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P04271

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins