Recombinant SOX9 antibody (AA 393-508)

Catalog No. ABIN7470674

This Rabbit Monoclonal antibody specifically detects SOX9 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant SOX9 antibody (AA 393-508) (ABIN7470674)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 709R

Product Details anti-SOX9 Antibody

Binding Specificity: AA 393-508

Purpose: SOX9 antibody (709R)

Immunogen: Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.

Product Description: SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date: 24 months

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background: Gene Alias: Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development