Recombinant Thyroglobulin antibody
Quick Overview for Recombinant Thyroglobulin antibody (ABIN7477599)
Target
See all Thyroglobulin (TG) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Isotype
- IgG
-
-
-
-
Application Notes
- IHC 1:100-1:200
-
Comment
-
Positive Control: Thyroid: A strong staining is seen in follicular cells and the colloid. The stroma is typically also stained due to a contamination artifact.
Negative Control: Colon: All cells must not show any staining.
-
Protocol
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Thyroglobulin (TG)
-
Alternative Name
- Thyroglobulin
-
Background
- AITD3, hTG, TDH3, Tg, Tgn,Thyroglobulin antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P01266
-
Pathways
- Thyroid Hormone Synthesis
Target
-