Recombinant TIM3 antibody

Catalog No. ABIN7477601

The Rabbit Monoclonal anti-TIM3 antibody has been validated for IHC. It is suitable to detect TIM3 in samples from Human.

Quick Overview for Recombinant TIM3 antibody (ABIN7477601)

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-366R

Product Details anti-TIM3 Antibody

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Tonsil: A moderate to strong TIM-3 immunostaining should be seen in a significant fraction of T-cells, while only few TIM-3 positive cells are seen in the germinal centre and the mantle zone.

Negative Control: Tonsil: Epithelial cells should be negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM3

Target Type: Virus

Background: CD366, HAVR2, Hepatitis A virus cellular receptor 2 (HAVCR2), Kidney injury molecule 3 (KIM3), T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM3, TIMD3,TIM3 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints