Recombinant NKX2-1 antibody

Catalog No. ABIN7477609

This anti-NKX2-1 antibody is a Rabbit Monoclonal antibody detecting NKX2-1 in IHC. Suitable for Human.

Quick Overview for Recombinant NKX2-1 antibody (ABIN7477609)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NKX2-1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-312R

Product Details anti-NKX2-1 Antibody

Immunogen: Recombinant human TTF-1 protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Lung: Nuclear TTF1 staining should be strong in pneumocytes and in basal cells of the terminal bronchioles while a weaker, still moderate to strong staining should be seen in columnar epithelial cells of the terminal bronchioles (low expressor control).

Negative Control: Liver: TTF1 staining must not be seen in any cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Alternative Name: TTF-1 / Thyroid Transcription Factor 1 / NKX2-1

Background: BCH, Benign chorea, BHC, Homeobox protein Nkx2.1, NK2 homeobox 1, NKX2.1, NKX2A, TEBP, Thyroid nuclear factor-1, Thyroid specific enhancer binding protein, Thyroid transcription factor-1 (TTF-1), Tin man, TITF1, TTF-1,TTF-1 / Thyroid Transcription Factor 1 / NKX2-1 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour