Recombinant VWF antibody (AA 1815-1939)
Quick Overview for Recombinant VWF antibody (AA 1815-1939) (ABIN7470691)
Target
See all VWF AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- AA 1815-1939
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Purpose
- von Willebrand Factor / vWF antibody (521R)
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Immunogen
- Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
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Isotype
- IgG
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Application Notes
- IHC 1:50-1:100
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Comment
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Positive Control: Colon: A moderate to strong endothelial vWF immunostaining should be seen in a fraction of blood vessels.
Product Description: von Willebrand Factor / vWF antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: vWF immunostaining should be completely absent in epithelial and muscular cells.
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Protocol
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes.
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Restrictions
- For Research Use only
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Format
- Liquid
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Expiry Date
- 24 months
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- VWF (Von Willebrand Factor (VWF))
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Alternative Name
- von Willebrand Factor / vWF
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Background
- Gene Alias: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2,von Willebrand Disease (vWD)
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UniProt
- P04275
Target
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