VWF antibody (AA 1304-1452)

Catalog No. ABIN3042349

This Rabbit Polyclonal antibody specifically detects VWF in IHC, WB and IF. It exhibits reactivity toward Rat and Mouse. It has been mentioned in 17+ publications

Quick Overview for VWF antibody (AA 1304-1452) (ABIN3042349)

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VWF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-VWF Antibody

Binding Specificity: AA 1304-1452

Purpose: Anti-Von Willebrand Factor/VWF Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Von Willebrand Factor/VWF Antibody. Tested in IF, IHC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse VWF recombinant protein (Position: M1304-E1452).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat Immunofluorescence, 2 μg/mL, Rat

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-VWF antibody (ABIN3042349)

Zhang, Zhou, Zhou, Zhao, Chai, Liu, Zheng, Wang, Wang, Zhao: "Preparation of a nano- and micro-fibrous decellularized scaffold seeded with autologous mesenchymal stem cells for inguinal hernia repair." in: International journal of nanomedicine, Vol. 12, pp. 1441-1452, (2017) (PubMed).

Zhang, Zhao, Zhao, Gu, Peng, Pu, Wu: "Expression and correlation analysis of RegIV and vascular endothelial growth factors (VEGF-A and VEGF-C) in metastatic spinal tumors." in: Oncology letters, Vol. 13, Issue 5, pp. 3517-3521, (2017) (PubMed).

Zhang, Li, Zheng, Lin, Zhang, Zhang: "Inhibition of angiogenesis by arsenic trioxide via TSP-1-TGF-β1-CTGF-VEGF functional module in rheumatoid arthritis." in: Oncotarget, Vol. 8, Issue 43, pp. 73529-73546, (2017) (PubMed).

Mu, Song, Zhang, Lin, Dong: "Calcium signaling is implicated in the diffuse axonal injury of brain stem." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 4388-97, (2016) (PubMed).

Ding, Chen, Xu, Qin: "Effect of Hyperbaric Oxygen on the Growth of Intracranial Glioma in Rats." in: Chinese medical journal, Vol. 128, Issue 23, pp. 3197-203, (2016) (PubMed).

Pan, Yu, Huang, Zhu: "Resveratrol Protects against TNF-α-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147034, (2016) (PubMed).

Yang, Yang, Cao, Man, Li, Xing: "Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells." in: Molecular medicine reports, Vol. 11, Issue 3, pp. 1784-92, (2015) (PubMed).

Qi, Li, Zhang, Zhang, Wang, Zhang, Gui, Huang: "Correction: IFI6 Inhibits Apoptosis via Mitochondrial-Dependent Pathway in Dengue Virus 2 Infected Vascular Endothelial Cells." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138896, (2015) (PubMed).

Wang, Jiang, Wang, Li, Zhang, Zhao, Peng, Cao, Sun: "Construction of tissue-engineered bone using a bioreactor and platelet-rich plasma." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 413-418, (2014) (PubMed).

Zhu, Tuerxun, Hui, Abliz: "Effects of propranolol and isoproterenol on infantile hemangioma endothelial cells in vitro." in: Experimental and therapeutic medicine, Vol. 8, Issue 2, pp. 647-651, (2014) (PubMed).

Wu, Chen, Guo, He, Hu: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, (2014) (PubMed).

Han, Jia, Dai, Yang, Zhao, Zhao, Fan, Yuan: "Performance of a multilayered small-diameter vascular scaffold dual-loaded with VEGF and PDGF." in: Biomaterials, Vol. 34, Issue 30, pp. 7302-13, (2013) (PubMed).

Yang, Qin, Wang, Chen, Yuan, Fang, Yao, Jiao, Yu, Zhang, Wang: "Pinocembrin, a major flavonoid in propolis, improves the biological functions of EPCs derived from rat bone marrow through the PI3K-eNOS-NO signaling pathway." in: Cytotechnology, Vol. 65, Issue 4, pp. 541-51, (2013) (PubMed).

Liu, Zhang, Hou, Wang, Yang, Guan, Dong, Gao, Feng: "Vascularized bone tissue formation induced by fiber-reinforced scaffolds cultured with osteoblasts and endothelial cells." in: BioMed research international, Vol. 2013, pp. 854917, (2013) (PubMed).

Xu, Fu, Chen, Xiao, Lan, Le, Cheng, He, Shen, Huang, Liu: "Development of an optimized protocol for primary culture of smooth muscle cells from rat thoracic aortas." in: Cytotechnology, Vol. 61, Issue 1-2, pp. 65-72, (2010) (PubMed).

Zhao, Chen, Li, Xu: "PlexinA1 expression in gastric carcinoma and its relationship with tumor angiogenesis and proliferation." in: World journal of gastroenterology, Vol. 13, Issue 48, pp. 6558-61, (2007) (PubMed).

Jiang, Xu, Zhu, Chen, Qian, Qiao, Yang, Wang, Chen: "Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells." in: Oncology reports, Vol. 16, Issue 5, pp. 1021-8, (2006) (PubMed).

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: VWF

Background:

Background: Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Gene Full Name: Von Willebrand factor

Sequence Similarities: Contains 1 CTCK (C-terminal cystine knot-like) domain.

Molecular Weight: 309 kDa

Gene ID: 22371

UniProt: Q8CIZ8