Calpastatin antibody (AA 431-530)

Catalog No. ABIN720161

The Rabbit Polyclonal anti-Calpastatin antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect Calpastatin in samples from Human and Mouse. There is 1 publication available.

Quick Overview for Calpastatin antibody (AA 431-530) (ABIN720161)

Target: Calpastatin (CAST)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpastatin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Calpastatin Antibody

Binding Specificity: AA 431-530

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Calpastatin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Calpastatin antibody (ABIN720161)

Huang, Rose, Hoffmann, Hashimoto, Bertino, Denk, Takano, Iwata, Saido, Hoffmann: "Calpastatin prevents NF-?B-mediated hyperactivation of macrophages and attenuates colitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 7, pp. 3778-88, (2013) (PubMed).

Target Details for Calpastatin

Target: Calpastatin (CAST)

Alternative Name: Calpastatin

Background:

Synonyms: BS-17, Calpastatin, Calpain inhibitor, Sperm BS-17 component, CAST

Background: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

Gene ID: 831

UniProt: P20810