Calpastatin antibody (AA 431-530)
CAST
Reactivity: Human, Mouse
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
1 image
(1 reference)-
- Target See all Calpastatin (CAST) Antibodies
- Calpastatin (CAST)
- Binding Specificity
- AA 431-530
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human, Mouse
- Predicted Reactivity
- Rat,Cow
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Calpastatin
- Isotype
- IgG
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- Application Notes
-
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
-
-
: "Calpastatin prevents NF-?B-mediated hyperactivation of macrophages and attenuates colitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 7, pp. 3778-88, (2013) (PubMed).
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: "Calpastatin prevents NF-?B-mediated hyperactivation of macrophages and attenuates colitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 7, pp. 3778-88, (2013) (PubMed).
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- Target
- Calpastatin (CAST)
- Alternative Name
- Calpastatin
- Background
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Synonyms: BS-17, Calpastatin, Calpain inhibitor, Sperm BS-17 component, CAST
Background: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
- Gene ID
- 831
- UniProt
- P20810
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