E. Coli antibody (FITC)

Catalog No. ABIN7478173

This anti-E. Coli antibody is a Rabbit Polyclonal antibody detecting E. Coli in Func. Suitable for E. coli.

Quick Overview for E. Coli antibody (FITC) (ABIN7478173)

Target: E. Coli

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E. Coli antibody is conjugated to FITC

Application: Functional Studies (Func)

Product Details anti-E. Coli Antibody (FITC)

Specificity: All "O" and "K" antigenic serotypes of Escherichia coli

Cross-Reactivity (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: Mixtures of all antigenic serotypes

Isotype: IgG

Application Details

Application Notes: TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). Will remove E. coli proteins from recombinant preparations

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Target Details for E. Coli

Target: E. Coli

Target Type: Bacteria