E. Coli antibody (Peroxidase (POD))

Catalog No. ABIN7478174

This Rabbit Polyclonal antibody specifically detects E. Coli in EIA, WB and IHC. It exhibits reactivity toward E. coli.

Quick Overview for E. Coli antibody (Peroxidase (POD)) (ABIN7478174)

Target: E. Coli

Reactivity: E. coli

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E. Coli antibody is conjugated to Peroxidase (POD)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-E. Coli Antibody (Peroxidase (POD))

Specificity: All "O" and "K" antigenic serotypes of Escherichia coli

Cross-Reactivity (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Mixtures of all antigenic serotypes

Isotype: IgG

Application Details

Application Notes: TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays. Will remove E. coli proteins from recombinant preparations

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for E. Coli

Target: E. Coli

Target Type: Bacteria