E. Coli antibody (FITC)

Catalog No. ABIN7478177

This Goat Polyclonal antibody specifically detects E. Coli in Func. It exhibits reactivity toward E. coli.

Quick Overview for E. Coli antibody (FITC) (ABIN7478177)

Target: E. Coli

Reactivity: E. coli

Host: Goat

Clonality: Polyclonal

Conjugate: This E. Coli antibody is conjugated to FITC

Application: Functional Studies (Func)

Product Details anti-E. Coli Antibody (FITC)

Specificity: Many "O" and "K" antigenic serotypes of Escherichia coli

Cross-Reactivity (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: Mixture of E. coli serotypes

Isotype: IgG

Application Details

Application Notes: TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Target Details for E. Coli

Target: E. Coli

Target Type: Bacteria