The Goat Polyclonal anti-Elementary Bodies antibody has been validated for IHC, WB and EIA. It is suitable to detect Elementary Bodies in samples from Chlamydia trachomatis.
Catalog No. ABIN7478217
Quick Overview for Elementary Bodies antibody (Peroxidase (POD)) (ABIN7478217)
This Elementary Bodies antibody is conjugated to Peroxidase (POD)
Application
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Specificity
Purified elementary bodies, disrupted
Cross-Reactivity (Details)
Cross-reacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and egg yolk sac
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
EB
Reactivity: Chlamydia trachomatis
IF
Host: Goat
Polyclonal
HRP
Application Notes
TITER : >1:1,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3), This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.