The Rabbit Polyclonal anti-Treponema Pallidum antibody has been validated for WB, IHC and EIA. It is suitable to detect Treponema Pallidum in samples from Treponema pallidum.
Catalog No. ABIN7478253
Quick Overview for Treponema Pallidum antibody (Peroxidase (POD)) (ABIN7478253)
Target
Treponema Pallidum
Reactivity
Treponema pallidum
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Treponema Pallidum antibody is conjugated to Peroxidase (POD)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
Specificity
All antigens
Cross-Reactivity (Details)
This antiserum has not been absorbed & may react with related microorganisms
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
Reactivity: Treponema pallidum
IHC (p), IFA
Host: Rabbit
Polyclonal
FITC
Application Notes
TITER : >1:3,000 in indirect FA. Non-reactive in RPR assay. This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.