Clostridium Species antibody (Peroxidase (POD))

Catalog No. ABIN7478328

The Rabbit Polyclonal anti- antibody has been validated for WB, IHC and EIA. It is suitable to detect in samples from Clostridium.

Quick Overview for Clostridium Species antibody (Peroxidase (POD)) (ABIN7478328)

Target: Clostridium Species

Reactivity: Clostridium

Host: Rabbit

Clonality: Polyclonal

Conjugate: Peroxidase (POD)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Product Details

Specificity: Reactive with proteins from common Clostridia species

Cross-Reactivity (Details): Antiserum is not absorbed & may react with related microorganisms, Reactive (IFA) with Cl. perfingens, Cl. sordellii, Cl. novyi, Cl. septicum, Cl. chauvoei

Purification: This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Extract of Cl. perfingens, Cl. sordellii, Cl. novyi, Cl. septicum, Cl. chauvoei

Isotype: IgG

Application Details

Application Notes: TITER : >1:500 by indirect IFA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details

Target: Clostridium Species