COL3 antibody

Catalog No. ABIN7478854

This Rabbit Polyclonal antibody specifically detects COL3 in WB, ELISA, IF, IHC (p) and RIA. It exhibits reactivity toward Chicken.

Quick Overview for COL3 antibody (ABIN7478854)

Target: COL3 (Collagen, Type III (COL3))

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-COL3 Antibody

Cross-Reactivity: Cow, Human, Mouse, Rat

Cross-Reactivity (Details): Chicken, cross-reaction with human, bovine, mouse and rat collagen type III <0,1 %

Characteristics: Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Chicken collagen type III 100 % , chicken collagen type I <5 %.

Purification: Affinity purified antibody

Immunogen: Purified collagen type III from chicken skin

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Chicken skin or liver,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA 1:40, IHC(P) 1:500, ELISA > 1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Storage Comment: -20°C

Target Details for COL3

Target: COL3 (Collagen, Type III (COL3))

Alternative Name: Collagen Type III