COL3 antibody

Catalog No. ABIN7478855

The Rabbit Polyclonal anti-COL3 antibody has been validated for ELISA, IF, IHC (p) and RIA. It is suitable to detect COL3 in samples from Rat.

Quick Overview for COL3 antibody (ABIN7478855)

Target: COL3 (Collagen, Type III (COL3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3 antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Product Details anti-COL3 Antibody

Cross-Reactivity: Mouse

Cross-Reactivity (Details): Rat, cross-reaction with mouse

Characteristics: Type III collagen is an alpha1(III)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Rat collagen type III 100 % , rat collagen I <0.1 %, rat collagen type V <10 %, rat elastin <0.1 % (solid phase RIA at 1:50 dilution).

Purification: Affinity purified antibody

Immunogen: Purified collagen type III from rat skin

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Rat skin or liver,IHC(P), IFA, ELISA, RIA,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Lyophilized from phosphate buffered solution, no BSA and preservative added!

Preservative: Without preservative

Storage: -20 °C

Storage Comment: -20°C

Target Details for COL3

Target: COL3 (Collagen, Type III (COL3))

Alternative Name: Collagen Type III