Recombinant CCR5 antibody

Catalog No. ABIN7487682

The Rabbit Monoclonal anti-CCR5 antibody is suitable to detect CCR5 in samples from Human. It has been validated for ELISA, FACS, Dep and SPR.

Quick Overview for Recombinant CCR5 antibody (ABIN7487682)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Depletion (Dep), Surface Plasmon Resonance (SPR)

Clone: ST6

Product Details anti-CCR5 Antibody

Purpose: Anti-CCR5 [ST6], Rabbit IgG, kappa

Specificity: This antibody is specific for the YTSE region of the N-terminal extracellular domain of human CCR5.It also binds to rhesus CCR5.

Cross-Reactivity: Rhesus Monkey

Characteristics:

Original Species of Ab: Rabbit

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: This antibody was isolated from a phage displayed antibody fragment library. The library was generated from a New Zealand White rabbit immunised with purified CCR5-N-GST protein, and four rounds of panning were completed against immobilised CCR5-N-MBP antigen.

Isotype: IgG kappa

Application Details

Application Notes: When expressed intracellularly, this antibody is able to inhibit the surface expression of human CCR5, conferring T-lymphocytes with immunity to CCR5-dependent HIV-1 infection (Steinberger et al, 2000). Humanised ST6 (ST6/34) also has the same depleting effect (Steinberger et al, 2000). The high affinity of this antibody for CCR5-N-MBP has been confirmed in surface plasmon resonance analysis (Steinberg et al, 2000). This antibody has been used to stain CCR5-expressing cells in flow cytometry, with no staining of control cells observed (Steinberger et al, 2000).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background: CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, B8LFP0

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size