Recombinant CD163 antibody

Catalog No. ABIN7487686

This anti-CD163 antibody is a Rabbit Chimeric antibody detecting CD163 in FACS, ELISA and IF. Suitable for Pig.

Quick Overview for Recombinant CD163 antibody (ABIN7487686)

Target: CD163

Antibody Type: Recombinant Antibody

Reactivity: Pig

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD163 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Clone: 2A10

Product Details anti-CD163 Antibody

Purpose: Anti-CD163 [2A10 (2A10/11)], Rabbit IgG, lambda

Specificity: This antibody recognizes and binds porcine CD163.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.

Isotype: IgG lambda

Application Details

Application Notes: This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background: M130, sCD163, Scavenger receptor cysteine-rich type 1 protein M130

UniProt: Q2VL90