Recombinant LY75/DEC-205 antibody

Catalog No. ABIN7487688

The Rat Monoclonal anti-LY75/DEC-205 antibody has been validated for WB, FACS, IHC and IF. It is suitable to detect LY75/DEC-205 in samples from Mouse.

Quick Overview for Recombinant LY75/DEC-205 antibody (ABIN7487688)

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LY75/DEC-205 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: NLDC145

Product Details anti-LY75/DEC-205 Antibody

Purpose: Anti-CD205 [NLDC145], Rat IgG2a, kappa

Specificity: This antibody is specific for murine DEC-205, a lectin endocytic receptor expressed at high levels by many dendritic cell subsets, where it mediates antigen uptake. The epitope recognised by this antibody is trypsin-sensitive.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Wistar rats with mouse lymph node stroma.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody has been used in IHC analysis of acetone-fixed mouse lymph node sections and cytospin preparations (Krall et al, 1986), and immunofluorescence analysis of epidermal sheets ( Krall et al, 1986).It has also been used in immunoblot experiments (Inaba et al, 1995) and in FC experiments to identify DEC-205-expressing cell subsets (Inaba et al, 1995, Hawiger et al, 2001).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for LY75/DEC-205

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Alternative Name: CD205

Background: Ly-75, NLDC-145, DEC205, DEC-205, Lymphocyte antigen 75, Ly-75

UniProt: Q60767