Recombinant CD8 antibody

Catalog No. ABIN7487708

This Rabbit Chimeric antibody specifically detects CD8 in FACS, IF, IHC and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD8 antibody (ABIN7487708)

Target: CD8

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: G10-1

Product Details anti-CD8 Antibody

Purpose: Anti-CD8 [G10-1], Rabbit IgG, kappa

Specificity: This antibody is specific for human CD8, and binds human CD8+ T cells. It also cross-reacts with non-human primate CD8, including that of Macaca nemestrina (Southern pig-tailed macaque).

Cross-Reactivity: Macaca nemestrina

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: Human CD8.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used to stain cells expressing CD8 by indirect immunofluorescence (Uckun et al, 1989) and in immunohistochemical analysis of aortic and iliac artery tissue from nonhuman primates (Masuda & Ross, 1989). It has also been employed in immunoprecipitation and gel electrophoresis experiments to investigate cell surface CD8 complexes (Ledbetter et al, 1985). A FITC-conjugated version of this antibody has been used to identify T cell populations in flow cytometry experiments (Chen et al, 2003). When conjugated to the ribosome-inhibitory protein ricin, this antibody acts as a potent CD8+ T cell-directed immunotoxin (Uckun et al, 1989).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: Cluster of differentiation 8, G10.1, Tp32