Recombinant PD-L1 antibody

Catalog No. ABIN7487752

This anti-PD-L1 antibody is a Rabbit Chimeric antibody detecting PD-L1 in FACS and BR. Suitable for Human.

Quick Overview for Recombinant PD-L1 antibody (ABIN7487752)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This PD-L1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: 332M7

Product Details anti-PD-L1 Antibody

Purpose: Anti-PD-L1 [332M7], Rabbit IgG, kappa

Specificity: This antibody strongly binds human and cynomolgus monkey PD-L1 and shows weak binding for mouse PD-L1. PD-L1 is highly expressed in many human tumors. . PD-L1 exerts an immune suppressive signal through binding to PD-1 and B7.1, and tumor expression of PD-L1 can mediate cancer immune evasion. Blocking this pathway, therefore provides a rationale for cancer immunotherapy.

Cross-Reactivity: Cynomolgus, Mouse

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original parental antibody 332M1 was generated by immunizing BALB/c mice with recombinant human PD-L1. Later on the humanized version of 332M1 was generated (332M7) by grafting the CDRs of the original mouse antibody onto human framework regions.

Isotype: IgG kappa

Application Details

Application Notes: The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a concentration of 10 μg/mL. The mouse version of this antibody was reported to inhibit patient derived melanoma tumor in tumor-bearing mice. There was also found an increase in the gene expression of CD8 and IFN-γ in tumor samples from mice treated with mouse version of this antibody (US20180346571).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: CD274, PDL1, B7-H1, B7H1, B7 homolog 1, hPD-L1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, Programmed cell death 1 ligand 1, Programmed death ligand 1

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints