Recombinant T Cell Receptor antibody

Catalog No. ABIN7487760

Product Details

Target: T Cell Receptor

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunohistochemistry (IHC)

Purpose: Anti-TCR [H57-597], Rabbit IgG, lambda

Specificity: MAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.

Characteristics:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Immunogen: Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.

Clone: H57-597

Isotype: IgG lambda

Application Details

Application Notes: In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: T Cell Receptor

Alternative Name: TCR

Background: TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti

UniProt: P01852