Cyclin B1 antibody (PE)

Catalog No. ABIN7505971

The Mouse Monoclonal anti-Cyclin B1 antibody has been validated for ICFC. It is suitable to detect Cyclin B1 in samples from Mammalian.

Quick Overview for Cyclin B1 antibody (PE) (ABIN7505971)

Target: Cyclin B1 (CCNB1)

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: V152

Product Details anti-Cyclin B1 Antibody (PE)

Purpose: Anti-Cyclin B1 PE

Specificity: The mouse monoclonal antibody V152 recognizes cyclin B1, a 48 kDa intracellular protein necessary for G2/M phase transition of the cell cycle.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant His-tagged hamster cyclin B1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background: Cyclin B1,Cyclin B1 is a regulatory protein involved in mitosis. It complexes with p34(cdc2) to form maturation-promoting factor (MPF), and is necessary for proper control of the G2/M phase transition of the cell cycle. It is expressed in tissues containing proliferating cells, such as lymph node, testis et al.

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase