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Recombinant PKC alpha antibody (pThr638)
This Rabbit Monoclonal antibody specifically detects PKC alpha in WB, IP and IHC (p). It exhibits reactivity toward Human.
Quick Overview for Recombinant PKC alpha antibody (pThr638) (ABIN7516217)
Target
See all PKC alpha (PKCa) Antibodies
PKC alpha (PKCa)
(Protein Kinase C, alpha (PKCa))
Antibody Type
Recombinant Antibody
Reactivity
All reactivities for PKC alpha antibodies
Human
Host
All hosts for PKC alpha antibodies
Rabbit
Clonality
All clonalities for PKC alpha antibodies
Monoclonal
Conjugate
All conjugates for PKC alpha antibodies
This PKC alpha antibody is un-conjugated
Application
All applications for PKC alpha antibodies
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Clone
R09-7C7
Product Details anti-PKC alpha Antibody
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Binding Specificity
All epitopes for PKC alpha antibodies
pThr638
Purpose
Rabbit recombinant monoclonal antibody raised against synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PKC alpha.
Cross-Reactivity
Human
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PKC alpha
Isotype
IgG
Alternatives
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Application Details
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Application Notes
Immunohistochemistry (1:50-1:100),Immunoprecipitation (1:20),Western Blot (1:500-1:1,000),The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Handling
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Format
Liquid
Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA).
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Target Details for PKC alpha
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Target
PKC alpha (PKCa)
(Protein Kinase C, alpha (PKCa))
Alternative Name
PRKCA
Background
Protein kinase C, alpha
Gene ID
5578
Pathways
WNT Signaling , TCR Signaling , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Thyroid Hormone Synthesis , cAMP Metabolic Process , Myometrial Relaxation and Contraction , Cell-Cell Junction Organization , Regulation of G-Protein Coupled Receptor Protein Signaling , G-protein mediated Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Interaction of EGFR with phospholipase C-gamma , Thromboxane A2 Receptor Signaling , VEGFR1 Specific Signals , VEGF Signaling
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