Calpain antibody (AA 1-19)
This Mouse Monoclonal antibody specifically detects Calpain in WB. It exhibits reactivity toward Human.
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Catalog No. ABIN7538812
Quick Overview for Calpain antibody (AA 1-19) (ABIN7538812)
Target
Calpain
Reactivity
Human
Host
Mouse
Clonality
Monoclonal
Conjugate
This Calpain antibody is un-conjugated
Application
Western Blotting (WB)
Clone
Calp3d-2C4
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Binding Specificity
- AA 1-19
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Purpose
- Lyophilized Mouse Monoclonal Antibody Calpain
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Specificity
- This antibody reacts with full-size calpain 3 (94 kD) plus an additional fragment at 30 kD in human skeletal muscle. A band of 94 kD is seen with rabbit and dog muscle while extracts of hamster muscle show reactivity with the 94 kD band and a larger species of approximately 110 kD. This 110 kD band is the principal immunoreactive species seen in rat muscle extracts. This antibody produces no bands with mouse, pig or chicken muscle.
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Purification
- Tissue culture supernatant
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Immunogen
- Synthetic peptide containing amino acids 1-19 of the human calpain 3 sequence.
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Isotype
- IgG2b
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anti-Calpain (AA 396-555), (Catalytic Subunit) antibody
Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
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Application Notes
- Western Blotting: Typical working dilution 1:25-1:50. The optimal dilution should be determined by the user as calpain must be homogenized in treatment buffer
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with the volume of sterile distilled water indicated on the vial label.
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Buffer
- Lyophilized tissue culture supernatant containing 15 mM sodium azide.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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Storage Comment
- Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.
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Expiry Date
- 12 months
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- Calpain
Target
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