CD16 antibody

Catalog No. ABIN7538857

This Mouse Monoclonal antibody specifically detects CD16 in IHC, IHC (p) and HIER. It exhibits reactivity toward Human.

Quick Overview for CD16 antibody (ABIN7538857)

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: 2H7

Product Details anti-CD16 Antibody

Purpose: Liquid Mouse Monoclonal Antibody CD16

Specificity: Human CD16 antigen.

Characteristics: This product is intended for the qualitative identification by light microscopy of human CD16 antigen in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to the external domain of the CD16 molecule, common to both the transmembrane form and the GPI-linked form.

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:80 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 18.0 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process