IgG antibody

Catalog No. ABIN7538900

The Mouse Monoclonal anti-IgG antibody has been validated for IHC, HIER and IHC (p). It is suitable to detect IgG in samples from Human.

Quick Overview for IgG antibody (ABIN7538900)

Target: IgG

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgG antibody is un-conjugated

Application: Immunohistochemistry (IHC), Heat-induced Epitope Retrieval (HIER), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RWP49

Product Details anti-IgG Antibody

Purpose: Liquid Mouse Monoclonal Antibody Immunoglobulin G

Specificity: Human IgG.

Characteristics: This product is intended for the qualitative identification by light microscopy of human immunoglobulin G (IgG) molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to 327 amino acids of the human IgG molecule.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 59 mg/L

Buffer: This product is a liquid tissue culture supernatant containing 0.35 % ProClin™ 950 as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for IgG

Target: IgG

Alternative Name: Immunoglobulin G

Target Type: Antibody