Ki-67 antibody

Catalog No. ABIN7538903

The Mouse Monoclonal anti-Ki-67 antibody has been validated for IHC, IHC (p) and HIER. It is suitable to detect Ki-67 in samples from Human.

Quick Overview for Ki-67 antibody (ABIN7538903)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: MM1

Product Details anti-Ki-67 Antibody

Purpose: Liquid Mouse Monoclonal Antibody Ki67 Antigen

Specificity: Human Ki67 nuclear antigen expressed in all proliferating cells during late G1, S, M and G2 phases of the cell cycle.

Characteristics: This product is intended for the qualitative identification by light microscopy of Ki67 Antigen molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant fusion protein corresponding to a 1086 bp Ki67 motif-containing cDNA fragment.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 84 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki67 Antigen

Pathways: Glycosaminoglycan Metabolic Process