WT1 antibody (AA 1-181)

Catalog No. ABIN7538946

This anti-WT1 antibody is a Mouse Monoclonal antibody detecting WT1 in IHC, IHC (p) and HIER. Suitable for Human.

Quick Overview for WT1 antibody (AA 1-181) (ABIN7538946)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: WT49

Product Details anti-WT1 Antibody

Binding Specificity: AA 1-181

Purpose: Liquid Mouse Monoclonal Antibody Wilms' Tumor

Specificity: Human Wilms' Tumor WT1 gene product.

Characteristics: This product is intended for the qualitative identification by light microscopy of human Wilms' Tumor WT1 gene product in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: A prokaryotic recombinant protein containing 1-181 amino acids of the N-terminal of the Wilms' Tumor protein.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 9. Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 44 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms' Tumor

Pathways: Tube Formation