Topoisomerase I antibody

Catalog No. ABIN7539776

This anti-Topoisomerase I antibody is a Mouse Monoclonal antibody detecting Topoisomerase I in WB, ICC and FACS. Suitable for Human and Mouse.

Quick Overview for Topoisomerase I antibody (ABIN7539776)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3D4B1

Product Details anti-Topoisomerase I Antibody

Purpose: TOP1 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human TOP1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

FCM: 1/200 - 1/400

ICC: 1/100 - 1/400

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Molecular Weight: 90.7 kDa

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance