Recombinant CD31 antibody (Extracellular Domain) (Atto 565)

Catalog No. ABIN7540325

This anti-CD31 antibody is a Alpaca Multiclonal antibody detecting CD31 in IF, IF/ICC and IF (p). Suitable for Human.

Quick Overview for Recombinant CD31 antibody (Extracellular Domain) (Atto 565) (ABIN7540325)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Human

Host: Alpaca

Clonality: Multiclonal

Conjugate: This CD31 antibody is conjugated to Atto 565

Application: Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Clone: 2MAC4-2PEC47-3MAC24-3MAC69-3MAC82-3PEC92

Product Details anti-CD31 Antibody (Atto 565)

Binding Specificity: Extracellular Domain

Expression System: E.coli

Purpose: Alpaca anti-human CD31 VHH is a carefully developed and validated nanobody (single-domain antibody) binding to human CD31 (PECAM-1) highly suitabe for tissue staining.

Brand: LIMAAbody®

Specificity: Detects endogenous levels of total human CD31 protein.

Cross-Reactivity (Details): does not cross-react with mouse CD31 based on qualitative analysis

Characteristics: VHH protein tag: C-terminal (6x) His-Tag

Purification: Affinity-purified antibody fragment.

Immunogen: Recombinant protein containing the extracellular domain of murine CD31 protein.

Application Details

Application Notes: Immunofluorescence: 10 μg/mL (frozen sections), 5 μg/mL (cell culture), 20 μg/mL (wholemount)

Comment:

VHH protein tag: C-terminal (6x) His-Tag

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Expiry Date: 6 months

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: CD31, also known as PECAM-1, is an adhesion molecule, which is expressed on endothelial cells at intercellular junctions and various T cell subsets. It is also found to be expressed on platelets, as well as most other leukocytes including monocytes and neutrophils. CD31 facilitates homotypic binding to itself, as well as heterotypic binding to the leukocyte integrins. CD31 plays a crucial role in facilitating the transendothelial migration of leukocytes through the intercellular junctions of vascular endothelial cells.

Molecular Weight: 82 kDa

NCBI Accession: NP_000433

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization