Recombinant CD8 antibody (Extracellular Domain) (Atto 565)

Catalog No. ABIN7540333

The Alpaca Monoclonal anti-CD8 antibody has been validated for FACS, IF, IF (p) and IF/ICC. It is suitable to detect CD8 in samples from Human.

Quick Overview for Recombinant CD8 antibody (Extracellular Domain) (Atto 565) (ABIN7540333)

Target: CD8

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Human

Host: Alpaca

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to Atto 565

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 3ALB177

Product Details anti-CD8 Antibody (Atto 565)

Binding Specificity: Extracellular Domain

Expression System: E.coli

Purpose: Anti-CD8 (Human) LIMAAbody® is a carefully developed and validated polyclonal nanobody (single-domain antibody) binding to human CD8 highly suitable for tissue stainings.

Brand: LIMAAbody®

Specificity: Detects endogenous levels of total human CD8 alpha protein.

Cross-Reactivity (Details): not analysed

Characteristics: VHH protein tag: C-terminal (6x) His-Tag

Purification: Affinity-purified antibody fragment.

Immunogen: Recombinant protein containing the extracellular domain of human CD8 protein.

Application Details

Application Notes: Immunofluorescence: 10 μg/mL (frozen sections), 10 μg/mL (cell culture), 20 μg/mL (wholemount), 10 μg/mL (FACS)

Comment:

VHH protein tag: C-terminal (6x) His-Tag

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Expiry Date: 6 months

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: CD8, known as Cluster of Differentiation 8, forms a disulphide-linked heterodimer comprising unrelated α and β subunits. Each subunit, characterized as a glycoprotein, is structured with a single extracellular Ig-like domain, a polypeptide linker, a transmembrane component, and a concise cytoplasmic tail. Within T cells, CD8 serves as the co-receptor alongside the T cell receptor (TCR). These distinct structures collaboratively identify the Antigen-Major Histocompatibility Complex (MHC). Specifically, the interaction occurs between the Ig-like domain of CD8α and the α3-domain of the MHC class I molecule. CD8 plays a pivotal role in ensuring the specificity of the TCR-antigen interaction, extending the duration of contact between the T cell and the antigen-presenting cell. Moreover, the α chain of CD8 recruits the tyrosine kinase Lck, an indispensable component for initiating T cell activation .

Molecular Weight: 26 kDa

NCBI Accession: NP_001759

UniProt: P01732