Galectin 3 antibody (APC)

Catalog No. ABIN7540407

The Rat Monoclonal anti-Galectin 3 antibody has been validated for FACS and ICFC. It is suitable to detect Galectin 3 in samples from Human and Mouse.

Quick Overview for Galectin 3 antibody (APC) (ABIN7540407)

Target: Galectin 3 (LGALS3)

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Galectin 3 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Clone: M3-38

Product Details anti-Galectin 3 Antibody (APC)

Purpose: Anti-Galectin-3 APC

Specificity: The rat monoclonal antibody M3/38 recognizes an epitope within amino acids 40-100 in N-terminal domain of murine galectin 3, a 35 kDa lectin expressed both intracellularly, and after its secretion also on the cell surface.

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: murine galectin-3

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for Galectin 3

Target: Galectin 3 (LGALS3)

Alternative Name: Galectin-3

Background:

NCBI Full Gene Name: lectin, galactose binding, soluble 3

Description: Galectin-3 is a galactose-binding lectin, which modulates intercellular interactions and interactions of the cell with ECM, as well as it is a nuclear protein and a component of inner mitochondrial membrane. Galectin-3 binds IgE, and takes part in formation of immunological synapse. It is detected cytoplasmatically in adenomas and carcinomas by immunohistochemistry. It is expressed in colonic and intestinal epithelium, papillary and follicular carcinomas, neoplastic astrocytes, inflammatory macrophages, and some lymphocytes. Upregulation of galectin-3 is involved in cancer progression and metastasis.

Other names: Mac-2, GBP, Gal-3, L-34

Gene name: Lgals3

Gene ID: 16854

UniProt: P16110

Pathways: RTK Signaling