GPR77 antibody (APC)

Catalog No. ABIN7540411

This anti-GPR77 antibody is a Mouse Monoclonal antibody detecting GPR77 in FACS. Suitable for Human and Non-Human Primate.

Quick Overview for GPR77 antibody (APC) (ABIN7540411)

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This GPR77 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 1D9-M12

Product Details anti-GPR77 Antibody (APC)

Purpose: Anti-Hu C5aR2 APC

Specificity: The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: L1.2 cells transfected with human C5aR2

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Alternative Name: C5aR2

Background:

NCBI Full Gene Name: complement component 5a receptor 2

Description: C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin). It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.

Other names: C5L2, GPR77, GPF77

Gene name: C5AR2

Gene ID: 27202

UniProt: Q9P296