MCAM antibody (APC)

Catalog No. ABIN7540418

This Mouse Monoclonal antibody specifically detects MCAM in FACS. It exhibits reactivity toward Human, Mouse, Rabbit and Dog.

Quick Overview for MCAM antibody (APC) (ABIN7540418)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Mouse, Rabbit, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: P1H12

Product Details anti-MCAM Antibody (APC)

Purpose: Anti-Hu CD146 APC

Specificity: The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

No Cross-Reactivity: Rat

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: cultured human umbilical cells

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background:

NCBI Full Gene Name: melanoma cell adhesion molecule

Description: CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.

Other names: MelCAM, MCAM, MUC18

Gene name: MCAM

Gene ID: 4162

UniProt: P43121