RUNX1 antibody

Catalog No. ABIN7540477

Supplier Product No.: 100-401-v33

This anti-RUNX1 antibody is a Rabbit Polyclonal antibody detecting RUNX1 in WB, ELISA and ChIP. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for RUNX1 antibody (ABIN7540477)

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Product Details anti-RUNX1 Antibody

Supplier Product No.: 100-401-v33

Supplier: Rockland

Purpose: AML1-ETO Antibody

Cross-Reactivity (Details): This product reacts with human AML1-ETO.

Purification: Anti-AML1-ETO Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Sterility: Sterile filtered

Immunogen: Anti-AML1-ETO Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human AML1-ETO.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 4 μL/ChIP

ELISA_Dilution: 1:500

Western_Blot_Dilution: 1:1,000

Comment:

Suggested Applications: Other
Anti-AML1-ETO Antibody has been tested in ChIP, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 80 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RUNX1 antibody (ABIN7540477)

Qi, Zhang, Liu, Tang, Dai, Chen, Lin, Zhu, Li: "HDN-1 induces cell differentiation toward apoptosis in promyelocytic leukemia cells depending on its selective effect on client proteins of Hsp90." in: Toxicology and applied pharmacology, Vol. 417, pp. 115459, (2021) (PubMed).

Target Details for RUNX1

Target: RUNX1 (Runt-Related Transcription Factor 1 (RUNX1))

Alternative Name: RUNX1

Background: Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit,This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22. This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukemia. It produces a chimerical gene made up of the 5'-region of AML1and the 3'-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Anti-AML1-ETO Antibody is ideal for research in Cancer and Gene Expression.

Gene ID: 861

NCBI Accession: NP_001001890

UniProt: Q01196