Gelsolin antibody

Catalog No. ABIN7540497

Supplier Product No.: 200-301-p37

The Mouse Monoclonal anti-Gelsolin antibody has been validated for WB, IHC and ELISA. It is suitable to detect Gelsolin in samples from Human.

Quick Overview for Gelsolin antibody (ABIN7540497)

Target: Gelsolin (GSN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: GS-2C4

Product Details anti-Gelsolin Antibody

Supplier Product No.: 200-301-p37

Supplier: Rockland

Purpose: Gelsolin Antibody

Cross-Reactivity (Details): This product reacts with human and rabbit.

Purification: Anti-Gelsolin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Gelsolin antibody was produced in mice by repeated immunizations with human plasma gelsolin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5-1 μg/mL

Comment:

Anti-Gelsolin is tested for Immunohistochemistry -P, -F and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~85.7 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: GSN

Background: AGEL, ADF, Brevin, Actin-depolymerizing factor,Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. This antibody is suitable for researchers interested in cell research and cytoskeletal signaling research.

Gene ID: 2934

NCBI Accession: NP_000168

UniProt: P06396

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy