SHANK3 antibody (AA 586-626) (PE)

Catalog No. ABIN7540509

Supplier Product No.: 200-308-w72

The Mouse Monoclonal anti-SHANK3 antibody has been validated for WB, IHC and ELISA. It is suitable to detect SHANK3 in samples from Rat.

Quick Overview for SHANK3 antibody (AA 586-626) (PE) (ABIN7540509)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SHANK3 antibody is conjugated to PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: S367-51

Product Details anti-SHANK3 Antibody (PE)

Binding Specificity: AA 586-626

Supplier Product No.: 200-308-w72

Supplier: Rockland

Purpose: SHANK1/SHANK3 Antibody Phycoerythrin

Cross-Reactivity (Details): BLAST analysis suggests that it is 100 % identical to mouse, 97 % identical to human and ~70 % identical to SHANK1 and SHANK2.

Purification: Anti-SHANK1/SHANK3 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-SHANK1/SHANK3 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 586-626 (SH3 domain) of rat SHANK3.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Other: ICC/IF: 1:100

Comment:

Anti-SHANK1/SHANK3 R-PE Conjugated Antibody is suitable for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~190kDa on specific lysates or tissues. It will cross react with SHANK1, but it will not cross react with SHANK2. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 12 months

Target Details for SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Alternative Name: Shank3

Background: Shank postsynaptic density protein, SH3 and multiple ankyrin repeat domains 3, Proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, , SH3 and multiple ankyrin repeat domains protein 3,SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Anti-SHANK1/SHANK3 is ideal for research in Neuroscience, including autism spectrum disorder, and Cell Adhesion.

Gene ID: 59312

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity