FLIP antibody (C-Term)

Catalog No. ABIN7540512

Supplier Product No.: 200-401-983

The Rabbit Polyclonal anti-FLIP antibody has been validated for WB, ELISA, IHC, IF and FM. It is suitable to detect FLIP in samples from Human.

Quick Overview for FLIP antibody (C-Term) (ABIN7540512)

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-FLIP Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-401-983

Supplier: Rockland

Purpose: FLIP Alpha Antibody

Purification: Anti-FLIP Antibody is affinity chromatography purified via peptide column. The product was purified from monospecific antiserum by DEAE ion exchange chromatography.

Sterility: Sterile filtered

Immunogen: FLIP Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of the human FLIPalpha/FLIP_L protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 0.5-1 μg/mL

Other: ICC: 10 μg/mL

Comment:

FLIP Antibody has been tested for use in ELISA, western blotting, Immunofluorescence, and Immunohistochemistry.  HeLa whole cell lysate can be used as a positive control in western blotting, and a band at approximately 55 kDa is expected. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.02 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FLIP

Target: FLIP (CFLAR) (CASP8 and FADD-Like Apoptosis Regulator (CFLAR))

Alternative Name: CFLAR

Background: C FLIP antibody, c FLIPL antibody, c FLIPR antibody, c FLIPS antibody, CASH antibody, CASP8 and FADD like apoptosis regulator antibody, CASP8 and FADD like apoptosis regulator precursor antibody, CASP8 antibody, CASP8AP1 antibody, FLIP-L, CLARP1, MRIT alpha-1, CASH alpha, I-FLICE 1, FLAME-1 gamma,Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands.  Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family.  Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family.  FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively.  The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP.  FLIP contains two death effector domains (DEDs) and a caspase-like domain.  FLIP interacts with adapter protein FADD and caspase-8 and -10, and potently inhibits apoptosis induced by all known death receptors.  Four splice variants of FLIPs have been identified and termed FLIP α, β, γ, and δ, respectively. Anti-FLIP antibody is ideal for investigators involved in Apoptosis, Cytokines and Growth factor research.

Gene ID: 8837, 187608571

UniProt: O15519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development